Volume 6, Issue 2, June 2020, Page: 31-38
In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model
Elizabeth Finbarrs Bello, Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Enugu State, Nigeria
Abireh Ifeanacho Ezeteonu, Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Enugu State, Nigeria
Ude Vincent, Department of Anatomy, Faculty of Basic Medical Sciences, Enugu State University of Science and Technology, Parklane, Enugu, Enugu State, Nigeria
Received: Feb. 27, 2020;       Accepted: Mar. 18, 2020;       Published: May 12, 2020
DOI: 10.11648/j.jdmp.20200602.11      View  296      Downloads  152
Abstract
The leaves of Eugenia uniflora are used as anti-inflammatory and analgesic in the treatment of tissue injury in traditional medicine. The study evaluates the anti-inflammatory, analgesic, hematological and histological effects of ethanol extract of E. uniflora. The anti-inflammatory effect was accessed in test groups given different doses (50, 100 and 200 mg/kg, p. o) of the extract, a control group (0.1 mL/kg, normal saline) and standard drug group (Ibuprofen 100mg/kg, p. o) on formalin- induced paw edema. Body weight, paw weight, thickness and volume were evaluated. Analgesic property was accessed on separate group of animals by acetic acid-induced writhing and formalin-induced paw licking tests. Thereafter, hematological parameters were estimated and sections of the edematous skin were processed and stained with H and E. The Eugenia uniflora decreased paw volume and thickness after 3hr. There was dose dependent significant (P<0.05) decrease in the number of abdominal writhing and insignificant reduction in the second or late phases of formalin-induced paw licking test group. Reduction in RBC, HB, PCV and increase in platelet, ESR, WBC were observed in the formalin-induced paw edema group. Histopathological assessment revealed aggregation of inflammatory cells in skin sections of the untreated group of the formalin-induced paw edema but a noticeable reversal in the extract and Ibuprofen treatment groups. These profiles indicate E. uniflora has potent anti-inflammatory and analgesic effects comparable to Ibuprofen a non steroidal anti-inflammatory drug (NSAIDs) and justifies it use in the treatment of inflammation and local tissue injury.
Keywords
Edema, Inflammation, Analgesic, E. uniflora, Ibuprofen, Formalin
To cite this article
Elizabeth Finbarrs Bello, Abireh Ifeanacho Ezeteonu, Ude Vincent, In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute – inflammation Rat Model, Journal of Diseases and Medicinal Plants. Vol. 6, No. 2, 2020, pp. 31-38. doi: 10.11648/j.jdmp.20200602.11
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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