Volume 6, Issue 3, September 2020, Page: 65-71
Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi
Paul Malaba Makoye, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Innocent John Daniel, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Mourice Nyangabo Mbunde, Department of Natural Products Development and Formulation, Institute of Traditional Medicine, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Nelson Enos Masota, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania; Institute for Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
Joseph Sempombe, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Veronica Mugoyela, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Received: Jul. 23, 2020;       Accepted: Aug. 4, 2020;       Published: Aug. 18, 2020
DOI: 10.11648/j.jdmp.20200603.12      View  120      Downloads  46
This study investigated the antibacterial activities of crude extracts of three Tanzanian plants; Sorindeia madagascariensis, Mucuna stans and Albizia harveyi, following reports on their ethnomedicinal applications and those of their related species. The reported ethnomedicinal applications of the selected plants include treatment of; tuberculosis, urinary tract infections and bacterial infections of the skin among other applications. Plant material were collected from Njombe, Iringa and Pwani regions of Tanzania. Phytochemical screening and bioautography were conducted as per adopted methods. Screening for antibacterial activity was done by broth microdilution assay against the standard and clinical isolates of bacteria. Phytochemical screening revealed the presence of phenolics, tannins, flavonoids, terpenoids and glycosides among the plant extracts. Antibacterial activity-study displayed weak to moderate antibacterial activities of the plant extracts, whereby S. madagascariensis leaf extract displayed the highest activity against; Staphylococcus aureus (ATCC 25923), clinical isolate of S. aureus and a methicillin-resistant S. aureus (MRSA) isolate, at a minimum inhibitory concentration (MIC) of 192±0.00 µg/mL. Bioautography of S. madagascariensis indicated this antibacterial activity to be associated with polar compounds. MICs observed due to M. stans ranged from 770 to 3080 µg/mL against all tested bacterial species whereas the observed MICs due to A. harveyi ranged from 1283 to > 3080 µg/mL. These findings reveal the antibacterial activities of the selected plants, corroborating their ethnomedicinal applications. Bioautography-guided isolation of compounds from these plants particularly S. madagascariensis, may give leads for newer antibacterial agents.
Phytochemical Screening, Antibacterial Activity, Bioautography, S. madagascariensis, M. stans, A. harveyi
To cite this article
Paul Malaba Makoye, Innocent John Daniel, Mourice Nyangabo Mbunde, Nelson Enos Masota, Joseph Sempombe, Veronica Mugoyela, Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi, Journal of Diseases and Medicinal Plants. Vol. 6, No. 3, 2020, pp. 65-71. doi: 10.11648/j.jdmp.20200603.12
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This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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