Volume 6, Issue 3, September 2020, Page: 65-71
Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi
Paul Malaba Makoye, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Innocent John Daniel, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Mourice Nyangabo Mbunde, Department of Natural Products Development and Formulation, Institute of Traditional Medicine, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Nelson Enos Masota, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania; Institute for Pharmacy and Food Chemistry, University of Wurzburg, Wurzburg, Germany
Joseph Sempombe, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Veronica Mugoyela, Department of Medicinal Chemistry, School of Pharmacy, Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania
Received: Jul. 23, 2020;       Accepted: Aug. 4, 2020;       Published: Aug. 18, 2020
DOI: 10.11648/j.jdmp.20200603.12      View  120      Downloads  46
Abstract
This study investigated the antibacterial activities of crude extracts of three Tanzanian plants; Sorindeia madagascariensis, Mucuna stans and Albizia harveyi, following reports on their ethnomedicinal applications and those of their related species. The reported ethnomedicinal applications of the selected plants include treatment of; tuberculosis, urinary tract infections and bacterial infections of the skin among other applications. Plant material were collected from Njombe, Iringa and Pwani regions of Tanzania. Phytochemical screening and bioautography were conducted as per adopted methods. Screening for antibacterial activity was done by broth microdilution assay against the standard and clinical isolates of bacteria. Phytochemical screening revealed the presence of phenolics, tannins, flavonoids, terpenoids and glycosides among the plant extracts. Antibacterial activity-study displayed weak to moderate antibacterial activities of the plant extracts, whereby S. madagascariensis leaf extract displayed the highest activity against; Staphylococcus aureus (ATCC 25923), clinical isolate of S. aureus and a methicillin-resistant S. aureus (MRSA) isolate, at a minimum inhibitory concentration (MIC) of 192±0.00 µg/mL. Bioautography of S. madagascariensis indicated this antibacterial activity to be associated with polar compounds. MICs observed due to M. stans ranged from 770 to 3080 µg/mL against all tested bacterial species whereas the observed MICs due to A. harveyi ranged from 1283 to > 3080 µg/mL. These findings reveal the antibacterial activities of the selected plants, corroborating their ethnomedicinal applications. Bioautography-guided isolation of compounds from these plants particularly S. madagascariensis, may give leads for newer antibacterial agents.
Keywords
Phytochemical Screening, Antibacterial Activity, Bioautography, S. madagascariensis, M. stans, A. harveyi
To cite this article
Paul Malaba Makoye, Innocent John Daniel, Mourice Nyangabo Mbunde, Nelson Enos Masota, Joseph Sempombe, Veronica Mugoyela, Phytochemical Screening, Antibacterial Activity and Bioautography of Sorindeia madagascariensis, Mucuna stans, and Albizia harveyi, Journal of Diseases and Medicinal Plants. Vol. 6, No. 3, 2020, pp. 65-71. doi: 10.11648/j.jdmp.20200603.12
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
WHO. Global Health Estimates 2016: Disease burden by Cause, Age, Sex, by Country and by Region, 2000-2016. 2018. https://www.who.int/healthinfo/global_burden_disease/en/.
[2]
WHO. High levels of antibiotic resistance found worldwide, new data shows. 2018. https://www.who.int/mediacentre/news/releases/2018/antibiotic-resistance-found/en/.
[3]
The Review on Antimicrobial Resistance. Securing New Drugs for Future Generations: The Pipeline of Antibiotics. London; 2015.
[4]
Reardon S. Resistance to last-ditch antibiotic has spread farther than anticipated. Nature. 2017. https://www.nature.com/news/resistance-to-last-ditch-antibiotic-has-spread-farther-than-anticipated-1.22140.
[5]
WHO. Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. 2017; 5-7.
[6]
Elham B, Fawzia A. Colistin resistance in Acinetobacter baumannii isolated from critically ill patients : clinical characteristics, antimicrobial susceptibility and outcome. Afr Health Sci. 2019; 19 (3): 2400-6.
[7]
The Review on Antimicrobial Resistance. Tackling drug resistant infections globally: Final report and recommendations. Vol. 136. London; 2016.
[8]
WHO. Global antimicrobial resistance surveillance system report. 2018. https://www.who.int/glass/resources/publications/early-implementation-report/en/.
[9]
Jonas OB, Irwin A, Berthe FCJ, Le Gall FG, Marquez P V. Drug-resistant infections: a threat to our economic future (Vol. 2): final report (English). Washngton, D. C; 2017. http://documents.worldbank.org/curated/en/323311493396993758/final-report.
[10]
Rates SMK. Plants as source of drugs. Toxicon. 2001; 39 (5): 603-13.
[11]
Savoia D. Plant-derived antimicrobial compounds: Alternatives to antibiotics. Future Microbiol. 2012; 7 (8): 979-90.
[12]
Abdallah EM. Plants: An alternative source for antimicrobials. J Appl Pharm Sci. 2011; 1 (6): 16-20.
[13]
Mbunde MVN, Innocent E, Mabiki F, Andersson PG. Ethnobotanical survey and toxicity evaluation of medicinal plants used for fungal remedy in the southern highlands of Tanzania. J Intercult Ethnopharmacol. 2017; 6 (1): 84-96.
[14]
Lampariello LR, Cortelazzo A, Guerranti R, Sticozzi C, Valacchi G. The Magic Velvet Bean of Mucuna pruriens. J Tradit Complement Med. 2012; 2 (4): 331-9.
[15]
Salau AO, Odeleye OM. Antimicrobial activity of Mucuna pruriens on selected bacteria. African J Biotechnol. 2007; 6 (18): 2091-2.
[16]
Ifeanyi OE, Stanley MC, Chinedum OK, Chinwe IA, Emmanuel O, Nwamaka ON. Antimicrobial Activities of Mucuna pruriens (Agbara) on Some Human Pathogens. IOSR J Pharm Biol Sci. 2014; 9 (2): 09-13.
[17]
Tiwari P, Goyal A. Phytochemistry and pharmacological activity of Mucuna pruriens: A review. Int J Green Pharm. 2018; 12 (4): 237-41.
[18]
Kokila K, Priyadharshini SD, Sujatha V. Phytochemical properties of Albizia species: A review. Int J Pharm Pharm Sci. 2013; 5 (3): 70-3.
[19]
Donkeng Donfack FV, Roque S, Trigo G, Valere P, Fokou T, Rachel L, et al. Antimycobacterial activity of selected medicinal plants extracts from Cameroon. Int J Biol Chem Sci. 2014; 8 (February): 273-88.
[20]
Breteler FJ. The African genus Sorindeia (Anacardiaceae): A synoptic revision. 1999; 93-113.
[21]
Lovett JC, Ruffo CK, Gereau RE. Field Guide to the Moist Forest Trees of Tanzania. 1994; 1-193.
[22]
Mastan SA, Janaki Ramayya P, Mutyala Naidu L, Mallikarjuna K. Antimicrobial activity of various extracts of Mucuna pruriens leaves. Biomed Pharmacol J. 2009; 2 (1): 55-60.
[23]
Rezk A, Nolzen J, Schepker H, Albach DC, Brix K, Ullrich MS. Phylogenetic spectrum and analysis of antibacterial activities of leaf extracts from plants of the genus Rhododendron. BMC Complement Altern Med. 2015; 15 (1): 1-10.
[24]
Evans WC. Trease and Evans Pharmacognosy. 16th ed. Evans WC, Evans D, editors. Edinburgh: Elsevier Inc.; 2009. 135-147 p.
[25]
Shah BN, Seth AK. Textbook of Pharmacognosy and Phytochemistry. 1st ed. Chauhan SK, Goswami N, editors. New Delhi: Elsevier; 2013. 157-433 p.
[26]
Yeo YL, Chia YY, Lee CH, Sow HS, Yap WS. Effectiveness of maceration periods with different extraction solvents on in-vitro antimicrobial activity from fruit of Momordica charantia L. J Appl Pharm Sci. 2014; 4 (10): 16-23.
[27]
Bandar H, Hijazi A, Rammal H, Hachem A, Saad Z, Badran B. Techniques for the Extraction of Bioactive Compounds from Lebanese Urtica dioica. Am J Phytomedicine Clin Ther. 2013; 1 (6): 507-13.
[28]
Al-Mughrabi KI. Antimicrobial activity of extracts from leaves, stems and flowers of Euphorbia macroclada against plant pathogenic fungi. Phytopathol Mediterr. 2003; 42 (3): 245-50.
[29]
Akinyemi KO, Oluwa OK, Omomigbehin EO. Antimicrobial activity of crude extracts of the three medicinal plants used in South-West Nigerian folk medicine on some food borne bacterial pathogens. African J Tradit Complement Altern Med. 2006; 3 (4): 13-22.
[30]
Rao USM, Abdurrazak M, Mohd KS. Phytochemical screening, total flavonoid and phenolic content assays of various solvent extracts of tepal of Musa paradisiaca. Malaysian J Anal Sci. 2016; 20 (5): 1181-90.
[31]
Cavalieri SJ, Rankin ID, Harbeck RJ, Sautter RL, Mccarter YS, Sharp SE, et al. Manual of Antimicrobial Susceptibility Testing. Coyle MB, editor. 2005. 53-62 p.
[32]
European Committee for Antimicrobial Susceptibility Testing. Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution. Clin Microbiol Infect. 2003; 9 (8): 2-4.
[33]
Bayer Health Care. Medication guide for Ciprofloxacin hydrochloride. 2011. https://www.accessdata.fda.gov/drugsatfda_docs/label/2011/019537s075,020780s033lbl.pdf.
[34]
Kuete V. Potential of Cameroonian Plants and Derived Products against Microbial Infections: A Review. 2010; 1479-91.
[35]
Ríos JL, Recio MC. Medicinal plants and antimicrobial activity. J Ethnopharmacol. 2005; 100 (1-2): 80-4.
[36]
Pisano MB, Cosentino S, Viale S, Spanò D, Corona A, Esposito F, et al. Biological activities of aerial parts extracts of Euphorbia characias. Biomed Res Int. 2016; 2016.
[37]
Sigma-Aldrich. 58030 Iodonitrotetrazolium chloride (p-Iodonitrotetrazolium Violet, 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, INT). 2018.
[38]
Dewanjee S, Gangopadhyay M, Bhattacharya N, Khanra R, Dua TK. Bioautography and its scope in the field of natural product chemistry. J Pharm Anal. 2015; 5 (2): 75-84.
[39]
Choma IM, Grzelak EM. Bioautography detection in thin-layer chromatography. J Chromatogr A. 2011; 1218 (19): 2684-91. http://dx.doi.org/10.1016/j.chroma.2010.12.069.
[40]
Patil NN, Waghmode MS, Gaikwad PS, Gajbhai MH, Bankar A V. Bioautography guided Screening of Antimicrobial Compounds Produced by Microbispora V2. Int Res J Biol Sci. 2013; 2 (2): 65-8.
[41]
Amugune BK, Mwangi JW, Thoithi GN, Kibwage IO. In Vitro Screening of Ten Selected Traditionally Used Medicinal Plants in Vihiga County, Kenya for Antibacterial and Antifungal Activity. Int J Med Plants Nat Prod. 2017; 3 (2): 37-44.
[42]
Suleiman MM, McGaw LJ, Naidoo V, Eloff JN. Detection of antimicrobial compounds by bioautography of different extracts of leaves of selected South African tree species. African J Tradit Complement Altern Med. 2010; 7 (1): 64-78.
[43]
Noudogbessi JP, Alitonou GA, Avlessi F, Figueredo G, Chalard P, Chalchat JC, et al. Physical characteristics and Chemical compositions of Leaves extracts of Sorindeia grandifolia Engl. (Anacardiaceae) harvested at Kato, Benin. 2013; 2 (3): 31-5.
[44]
Fokou PVT, Kissi-Twum AA, Yeboah-Manu D, Appiah-Opong R, Addo P, Yamthe LRT, et al. In Vitro Activity of Selected West African Medicinal Plants against Mycobacterium ulcerans Disease. Molecules. 2016; 21 (4): 1-13.
[45]
Kamkumo RG, Ngoutane AM, Tchokouaha LR, Fokou PV, Madiesse EA, Legac J, et al. Compounds from Sorindeia juglandifolia (Anacardiaceae) exhibit potent anti-plasmodial activities in vitro and in vivo. Malar J. 2012; 11: 1-7.
[46]
Kumar P, Saha S. An updated review on Taxonomy, Phytochemistry, Pharmacology and Toxicology of Macuna pruriens. IC J J Pharmacogn Phytochem. 2013; 8192 (1): 2668735-5.
[47]
Murthy SN, Sangvikar S, Malgaonkar MM, Sharma C, Kulkarni YR. In vitro Physico-chemical, Phytochemical and Fluroscence Assessment of Mucuna species. 2016; 2 (2): 1-10.
[48]
Karunanithi M, David Raj C, Jegadeesan M, Kavimani S. Phytochemical Analysis and Comparative Aphrodisiac Activity of Four Species of Mucuna. Phytopharm Drug Deliv Approaches. 2019; 02-29.
[49]
Pandey J, Pandey R, Materials AP. Phytochemical study of seeds of Mucuna pruriens (L.) DC. 2016; 4 (I): 83-6.
[50]
Lorenzetti F, Macisaac S, Arnason JT, Buckles D. The phytochemistry, toxicology, and food potential of velvetbean (Mucuna spp., Fabaceae). Africa (Lond). 2010; 1-11.
[51]
Krishnaveni M, Hariharan D. Phytochemical Analysis of Mucuna pruriens and Hyoscyamus niger Seeds. 2017; 7 (2): 6-13.
[52]
El-Ghany AEA, Dora G, Abdallah RH, Hassan WHB, El-Salam EA. Phytochemical and biological study of Albizia lebbeck stem bark. J Chem Pharm Res. 2015; 7 (5): 29-43.
[53]
Lawan SA, Saleh A, Sani BG, Fa’iza AM, Sadiya AZ. Preliminary phytochemical constituents and phytotoxic effect of Albizia lebbeck (L.) Benth on Sorghum bicolor. Bayero J Pure Appl Sci. 2018; 10 (1): 405.
[54]
Bahgat D SA. Saponins from Genus Albizia: Phytochemical and Biological Review. Med Aromat Plants. 2015; s3: 1-7.
[55]
Taiwo O, Xu H-X, Lee SF. Antibacterial activities of extracts from Nigerian chewing sticks. Phyther Res. 1999; 13 (8): 675-9.
[56]
Rajalakshmi P V., Senthil K. Flavonoid content and antibacterial activity of Albizia julibrissin. Durazz leaf, Stem and flower extracts against clinically isolated bacterial pathogens. Int J Pharm Pharm Sci. 2014; 6 (11): 506-8.
[57]
Banothu V, Neelagiri C, Adepally U, Lingam J, Bommareddy K. Phytochemical screening and evaluation of in vitro antioxidant and antimicrobial activities of the indigenous medicinal plant Albizia odoratissima. Pharm Biol. 2017; 55 (1): 1155-61.
[58]
Abriham H, Paulos B. In vitro Antioxidant and Antibacterial Activity of Albizia lebbeck (L) Benth Stem Bark. Sci Technol Arts Res J. 2016; 4 (2): 204.
Browse journals by subject