Volume 6, Issue 3, September 2020, Page: 72-76
Comparative Study of the Phytochemistry and Antioxidant Activity of Anacardium occidentale (L.) Leaf and Stem Bark Extracts
Issiaka Togola, Département de Biologie, Faculté des Sciences et Techniques (FST), Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali
Youssouf Kaya, Département de Biologie, Faculté des Sciences et Techniques (FST), Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali
Nouhoum Diarra, Département de Biologie, Faculté des Sciences et Techniques (FST), Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali
Mamadou Abdoulaye Konare, Department of Pharmacognosy and Traditional Medicine, University of Jos, Jos, Nigeria
Adama Denou, Department of Pharmacognosy and Traditional Medicine, University of Jos, Jos, Nigeria; Department des Sciences Pharmaceutiques et de Médicine Traditionnelle, Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali
Rokia Sanogo, Department des Sciences Pharmaceutiques et de Médicine Traditionnelle, Université des Sciences, des Techniques et des Technologies de Bamako (USTTB), Bamako, Mali
Received: Aug. 31, 2020;       Accepted: Sep. 15, 2020;       Published: Sep. 23, 2020
DOI: 10.11648/j.jdmp.20200603.13      View  39      Downloads  23
Abstract
The use of plant roots and stem barks is a common practice in traditional medicine. This practice could lead to the disappearance of some plant species. Anacardium occidentale (L) is a plant whose stem bark is commonly used for the traditional management of diabetes and hypertension in Mali. In the hope of replacing this organ with the leaves, a comparative phytochemical study of the two organs was carried out. The Phytochemical screening was carried out through colouring and precipitation reactions. The Folin-Ciocalteu reagent was used to determine the content of total phenolic compounds whereas the flavonoids were determined using aluminium trichloride. Antioxidant activity was evaluated by the TAC and DPPH methods. The results obtained have shown that both organs were rich in secondary metabolites with a similar phytochemical profile. Also, it was found that regardless of the solvent used, the leaves contained the highest levels of total phenols and flavonoids. Thus, the contents of total phenols in methanolic extracts have been 211.2±21.8 mg GAE/g and 129.72±5.15 mg GAE/g for leaves and stem barks respectively. As for those of flavonoids, they have been 59.02±5.88 mg QE/g for leaves and 31.30±2.74 mg QE/g for stem barks. However, the stem barks showed the higher antioxidant activity than the leaves, which is also appreciable. In sum, other studies such as toxicological one must be conducted before replacing stem barks with leaves in the traditional management of these two pathologies.
Keywords
Anacardium occidentale, Phytochemistry, Antioxidant Activity, Leaf and Stem Bark
To cite this article
Issiaka Togola, Youssouf Kaya, Nouhoum Diarra, Mamadou Abdoulaye Konare, Adama Denou, Rokia Sanogo, Comparative Study of the Phytochemistry and Antioxidant Activity of Anacardium occidentale (L.) Leaf and Stem Bark Extracts, Journal of Diseases and Medicinal Plants. Vol. 6, No. 3, 2020, pp. 72-76. doi: 10.11648/j.jdmp.20200603.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
Tra Bi F H, Irié G M, N’gaman K C C, Mohou C H B. 2008. Études de quelques plantes thérapeutiques utilisées dans le traitement de l’hypertension artérielle et du diabète: deux maladies émergentes en Côte d’Ivoire. Sciences & Nature. 5 (1): 39–48.
[2]
Houehanou C, Amidou S, Preux P M, Houinato D and Lacroix P. 2018. Hypertension artérielle (HTA) en Afrique subsaharienne. JMV-Journal de Médecine Vasculaire 43 (2).
[3]
Diop S N, Diédhiou D. 2015. Le diabète sucré en Afrique sub-saharienne: aspects épidémiologiques et socioéconomiques. Médecine des maladies Métaboliques. 9 (2): 123-129.
[4]
Menta I, Ba HO, Sanogo KM. Hypertension (HTA) among young people of 18 to 35 years old in cardiology department of Gabriel Touré university teaching hospital. World Journal of Cardiovascular Diseases. 2018; 8 (1): 11-17.
[5]
IDF DIABETES ATLAS, Ninth edition 2019/AFRIQUE.
[6]
Dongock D N, Bonyo A L, Mapongmestem P M, Bayegone E. 2018. Etude ethnobotanique et phytochimique des plantes médicinales utilisées dans le traitement des maladies cardiovasculaires à Moundou (Tchad). International Journal of Biological and Chemical Sciences. 12 (1), 203-216.
[7]
Jiofack T., Fokunang C., Guedje N. M., Kemeuze V., Fongnzossie E., Nkongmeneck B. A, Mapongmetsem P. M., and Tsabang, N. 2010. Ethnobotanical uses of medicinals plants of two ethnoecological regions of Cameroon. International Journal of Medicine and Medical Sciences 2 (3): 60-79.
[8]
Mpondo M. E., Dibong D. S., Priso R. J., Ngoye A., Ladoh Y C. F. 2012. État actuel de la médecine traditionnelle dans le système de santé des populations rurales et urbaines de Douala (Cameroun). Journal of Applied Biosciences 55: 4036–4045.
[9]
Nole T, Lionel T D W, Cedrix T F S and Gabriel AA. 2016. Ethnomedical and Ethnopharmacological Study of Plants Used For Potential Treatments of Diabetes and Arterial Hypertension by Indigenous People in Three Phytogeographic Regions of Cameroon. Diabetes Case Reports. 1: 110.
[10]
Bio A, Toyi S. S. M, Yoka J, Djego G. J, Awede B, Laleye A, Sinsin A. B. 2015. Contribution aux connaissances des principales plantes antihypertensives utilisées en médecine traditionnelle à Bassila (Bénin, Afrique de l’Ouest). Revue CAMES – Série Pharm. Méd. Trad. Afr.; 17 (2): 8-18.
[11]
Delvaux C, Sinsin B, Darchambeau F, Van Damme P. 2009 Recovery from bark harvesting of 12 medicinal tree species in Benin, West Africa. J. Appl. Ecol., 46, 703–712.
[12]
Mbinile S D, Munishi L K, Ngondya I B and Ndakidemi P A. 2020. Conservation and Management Challenges Facinga Medicinal Plant Zanthoxylum chalybeum in Simanjiro Area, Northern Tanzania. Sustainability. 12, 4140.
[13]
Harborne J. B, 1998. Phytochimical Methods: A guide to moderne techniques of plant analysis 3e ed.: chapman and hill. 1998. 303p.
[14]
Srivastava N, Chauhan A, Sharma B. 2012. Isolation and characterization of some phytochemicals from Indian traditional plants. Biotechnology Research International. (4): 549850.
[15]
Singleton, V. L.; Orthofer, R.; Lamuela-Raventos, R. M. 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu Reagent. Methods Enzymol. 299, 152-178.
[16]
Chang C, Yang M, Wen H, Chern J. 2002. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. J Food Drug Analysis. 10: 178-182.
[17]
Brand Williams W, Cuvelier ME, Berset C. 1995. Use of free radical method to evaluate anti-oxidant activity. Lebensmittel Wissenschaft and Technologie. 28 (1): 25-30.
[18]
Prieto P, Pineda M, Anguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex. Specific application to the determination of Vitamin E. Anal Biochem 1999; 269: 337-341.
[19]
Desai D, Raorane C, Patil S, Rajashri Gadgil and Patkar D. 2017. Anacardium occidentale: fountain of phytochemicals; the qualitative profiling. World Journal of Pharmaceutical Research. 6 (5): 585-592.
[20]
Tchikaya F O, Bantsielé G B, Kouakou-Siransy G, Datté J Y, Yapo P A, Zirihi N G, Offoumou M A. 2011. Anacardium occidentale Linn. (Anacardiaceae) stem bark extract induces hypotensive and cardio-inhibitory effects in experimental animal models. Afr J Tradit Complement Altern Med. 8 (4): 452-461.
[21]
Abulude, F. O., Ogunkoya, M. O. and Akinjagunla, Y. S. (2010) Phytochemical screening of leaves and stem of Cashew tree (Anacardium occidentate). Environmental Agricultural and Food Chemistry, 9, 815-819.
[22]
Ifesan BOT, Fashakin JF, Ebosele F, Oyerinde AS. 2013. Antioxidant and antimicrobial properties of selected plant leaves. European Journal of Medicinal Plant. 3 (3): 465-473.
[23]
Da Costa CDF, Herculano EA, Silva JCG, Paulino ET, Bernardino AC, Araújo-Júnior JX, Sant’ana AEG, Salvador MJ, Ribeiro ÊAN. 2018. Hypotensive, vasorelaxant and antihypertensive activities of the hexane extract of Anacardium occidentale linn. Arch Biol Sci. 70 (3): 459-68.
[24]
Luka CD, Tijjani H, Joel EB, Ezejiofor UL, Onwukike P. 2013. Hypoglycaemic Properties of Aqueous Extracts of Anacardium occidentale, Moringa oleifera, Vernonia amygdalina and Helianthus annuus: A Comparative Study on Some Biochemical Parameters in Diabetic Rats. International Journal of Pharmaceutical Science Invention. 2 (7): 16-22.
[25]
Tsabang N, Yedjou CG, Tsambang LWD, Tchinda AT, Donfagsiteli N, Agbor GA, Tchounwou PBB and Nkongmeneck BA. 2015. Treatment of Diabetes and/or Hypertension Using Medicinal Plants in Cameroon. Medicinal & Aromatic Plants. S2: 003.
[26]
Aponjolosun SB and Fasola RT. 2020. Phytochemical, Antimicrobial and Toxicity Evaluation of Anacardium occidentale Linn. Leaf Extracts. Tropical Journal of Natural Product Research. 4 (4): 113-122.
[27]
Ojezele MO and Agunbiade S. 2013. Phytochemical Constituents and Medicinal Properties of Different Extracts of Anacardium Occidentale and Psidium Guajava. Asian Journal of Biomedical and Pharmaceutical Sciences. 3 (16): 20-23.
[28]
Jaiswal YS, Tatke PA, Gabhe SY and Vaidya A. 2010. Antioxidant Activity of Various Extracts of Leaves of Anacardium Occidentale (Cashew). Research Journal of Pharmaceutical, Biological and Chemical Sciences. 1 (4): 112- 119.
[29]
Bajalan I., Mohammadi M., Alaei M., Pirbalouti A. G. 2016. Total phenolic and flavonoid contents and antioxidant activity of extracts from different populations of lavandin. Ind. Crops Prod. 87, 255–260.
[30]
Da Silva R. A., Liberio S A, Do Amaral F MM, Do Nascimento F R F, Torres L M B, Neto V M, Guerra R N M (2016). Antimicrobial and Antioxidant Activity of Anacardium occidentale L. Flowers in Comparison to Bark and Leaves Extracts. Journal of Biosciences and Medicines, 4, 87-99.
[31]
Sija SL, Athulya AS, Mahima MR, Vidhya A. 2019. Antioxidant and antimicrobial activity of different plant parts of Anacardium occidentale L. and Mangifera indica L.: a comparative study. International Journal of Pharmaceutical Sciences and Drug Research 2019; 11 (4): 111-115.
[32]
Salehi B, Gültekin-Özgüven M, Kirkin C, Özçelik B, Morais-Braga M F B, Carneiro JNP, Bezerra CF, Silva TG, Coutinho HDM, Amina B, Armstrong L, Selamoglu Z, Sevindik M, Yousaf Z, Sharifi-Rad J, Muddathir AM, Devkota HP, Martorell M, Jugran AK, Cho WC and Martins N. 2020. Antioxidant, Antimicrobial, and Anticancer Effects of Anacardium Plants: An Ethnopharmacological Perspective. Front. Endocrinol. 11: 295.
[33]
Ajileye OO, Obuotor EM, Akinkunmi EO, Aderogba MA. 2015. Isolation and characterization of antioxidant and Antimicrobial compounds from Anacardium occidentale L. (Anacardiaceae) leaf extract. Journal of King Saud University–Science. 27, 244–252.
[34]
Razali N, Razab R, Junit SM, Aziz AA. Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale). Food Chem. (2008) 111: 38–44.
[35]
Tan YP and Chiang CEW 2014. Antioxidant, antityrosinase and antibacterial properties of fresh and processed leaves of Anacardium occidentale and Piper betle. Food Bioscience. 6: 17-23.
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