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Indole-3-Carbinol Inhibits Laryngeal Cancer Growth Through Cell Cycle Arrest
Chenggang Mao,
Xiaochun Zhou,
Yidao Jiang,
Lijia Wan,
Zezhang Tao
Issue:
Volume 5, Issue 2, April 2019
Pages:
22-28
Received:
21 March 2019
Accepted:
5 May 2019
Published:
4 June 2019
Abstract: The growth of a variety of tumors are inhibited by indole-3-carbinol (I3C) obviously. But, its role in laryngeal cancer is not clear. The goal of this study was to research the probable roles that laryngeal cancer cell apoptosis and proliferation Hep-2 was influenced by I3C. I3C dose-dependently therapy obviously inhibited Hep-2cell proliferation, and, I3C promoted apoptosis and induced cell morphological changes at 100, 200, 300, 400 μM doses. We discovered that I3C shows anticancer effect through various signal pathways after Hep-2 cells I3C therapy. In Hep-2laryngeal cancer cell line, through decreasing cell cycle-related proteins that include cyclin D1, CDK6, CDK4, and pRb, G1 arrest was induced by I3C. Apart from this, BALB/c nude mice constructed tumor-bearing mouse models. BALB/c nude mice were divided into three groups: treated with I3C, untreated control group and pretreated with I3C. After 8 weeks treatment, the untreated control group developed bigger tumors compared to mice treated or pretreated with I3C, and in the tumors such as cyclin D1, CDK6, CDK4 and pRb cell cycle-related proteins were obviously decreased. Further, the study result showed there was no harmful side effect in the heart, liver and kidney of the I3C-treated nude mice. In conclusion, both in vivo and in vitro I3C inhibited proliferation and induced the Hep-2 cells apoptosis, and showed low toxicity to normal cells. By suppressing the expression of cyclin families and CDK, we deduce that I3C can inhibit the Hep-2 cells growth in vitro. On normal organs and tissues, the I3C had no toxic effects and was safe.
Abstract: The growth of a variety of tumors are inhibited by indole-3-carbinol (I3C) obviously. But, its role in laryngeal cancer is not clear. The goal of this study was to research the probable roles that laryngeal cancer cell apoptosis and proliferation Hep-2 was influenced by I3C. I3C dose-dependently therapy obviously inhibited Hep-2cell proliferation, ...
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Laser Irradiation Induces DNA Polymorphism and Alters Phytochemicals Compositions as well as Growth and Yield of Curcuma longa
Fadia El-Sherif,
Yun-Kiam Yap,
Hairul Islam Ibrahim
Issue:
Volume 5, Issue 2, April 2019
Pages:
29-38
Received:
15 April 2019
Accepted:
23 May 2019
Published:
5 June 2019
Abstract: Curcuma longa, commonly known as curcuma or turmeric is a perennial herbaceous plant native to Indian subcontinent and Southeast Asia. Its underground part, referred as rhizome, is used for vegetative propagation and source for remedies for various illnesses. Curcuminoid and volatile oils are the major active components found in turmeric, the yellow power obtained from the ground rhizome. He-Ne laser irradiation has been used as biostimulator device to enhance plant growth. In this study, we have pre-treated the curcuma rhizomes with 5, 10- or 15-min He-Ne laser irradiation. Laser pre-treated and non-treated rhizomes were then cultivated in greenhouse for 8 months. The growth parameters, photosynthetic pigment contents, rhizomes yield were measured. The phytochemical compositions of rhizomes from different treatment groups were measured by gas chromatography mass spectrometry and compared. Effects of laser on DNA polymorphism was assessed by ISSR markers. Our results indicated that laser pre-treatments on rhizomes enhanced curcuma plant height, number of roots and rhizomes, rhizomes diameters, and dried weight of leaves and rhizomes, as well as photosynthetic pigments. The enhancement effects are more prominent in 10 min treatment groups. In addition, laser treatment also alters the phytochemical compositions of ethanol extract from the rhizomes. Interestingly, our study has revealed that laser treatment had resulted in DNA polymorphism as shown from the analysis by ISSR markers.
Abstract: Curcuma longa, commonly known as curcuma or turmeric is a perennial herbaceous plant native to Indian subcontinent and Southeast Asia. Its underground part, referred as rhizome, is used for vegetative propagation and source for remedies for various illnesses. Curcuminoid and volatile oils are the major active components found in turmeric, the yello...
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Documentation of Major Poisonous Plant on Livestock in Ethiopia
Temesgen Kassa Getahun,
Wondimu Debash Beze
Issue:
Volume 5, Issue 2, April 2019
Pages:
39-44
Received:
26 February 2019
Accepted:
3 April 2019
Published:
20 June 2019
Abstract: The review was conducted with the objective of documenting potentially poisonous plants in Ethiopia. One of the major causes of economic loss in the livestock industry is Poisonous plants especially in the developing country including Ethiopia. In each year poisonous plant affect 3 to 5 percent of the cattle, sheep, goat, and horses that graze ranges. Major loss caused by these plants result from death of livestock, abortions, photosensitization, decreased production, emaciation, and birth defects and also they have other losses due to increased management costs associated with such things as fencing to prevent access of animals to these plants , altered grazing programs, and loss of forage due to removal of these plants. Livestock mostly exposed to toxic plants in dry and early rainy season when feed is in short supply. In this documentation 71 different plants identified from four different area of the country were collected from different governmental and non-governmental organizations and published papers. A more extensive survey is required to document all poisonous plants in the rangelands and to identify the major toxic principles in the different species possibly pharmacological activity. This documentation review the current knowledge on the identity of plants known to have poisoned livestock and research conducted into these toxic plants.
Abstract: The review was conducted with the objective of documenting potentially poisonous plants in Ethiopia. One of the major causes of economic loss in the livestock industry is Poisonous plants especially in the developing country including Ethiopia. In each year poisonous plant affect 3 to 5 percent of the cattle, sheep, goat, and horses that graze rang...
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